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Virginia Mayo (November 30, 1920, St. Louis, Missouri, United States – January 17, 2005, Los Angeles, California, United States) was an American film actress who played Lady Barbara Wellesley, Horatio Hornblower's primary love interest, in Captain Horatio Hornblower R.N.

After a short career in vaudeville, Mayo progressed to films and during the 1940s established herself as a supporting player in such films as The Best Years of Our Lives (1946) and White Heat (1949).

Mayo was only cast as Lady Barbara after a number of high profile British actresses were not free or interested. Gregory Peck's personal choice was Margaret Leighton, who was physically much closer to Lady Barbara as she is described in the novels.

She worked extensively during the 1950s, but after this her appearances were fewer. She worked occasionally until her final performance in 1997.

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